ENZ_GDH_003

Glutamate Dehydrogenase (GDH)

EC Number: 1.4.1.2 CAS Number: 9029-12-3 BRENDA IUBMB

High-performance glutamate dehydrogenase purified from a thermophilic microorganism.

High-performance glutamate dehydrogenase purified from a thermophilic microorganism

Microbial origin

Free from any animal or plant related contamination.

High activity

Maintains high activity in a wide range of temperatures.

High Thermal Stability

Maintains over 85% of its activity for 8 hours at 50°C.

Swissaustral GDH is more stable than GDH from bovine liver sources in a broad spectrum of temperatures.

Potential Applications

GDH enables rapid ammonium and glutamate assays for clinical diagnostics, monitors metabolites in fermented foods, and powers enzymatic biofuel cells.

Main Properties

Origin Thermophilic microorganism
Production Source Escherichia coli
Molecular Weight ~270 kDa
Activity ≥ 90 U/mg of protein
Unit Definition One unit is defined as the conversion of 1 µmol of α-ketoglutarate into glutamate, in 1 minute at 50°C at pH 8.0
Temperature Range 20–70°C (optimal: 50°C)
pH Range 7.0–8.5 (optimal: 8.0)
Other Components 0.05 M Tris Buffer pH 8 and 0.5 M NaCl
Storage Conditions At 20°C, it maintains the reported activity at least for 14 months.

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