ENZ_LAC_005

Laccase (LAC)

EC Number: 1.10.3.2 CAS Number: 80498-15-3 BRENDA IUBMB

Recombinant Microbial Laccase (LAC).

Recombinant Microbial Laccase (LAC)

Recombinant

Fast and sustainable production in a non-pathogenic strain of E. coli.

Microbial origin

Free from any animal or plant related contamination.

High Thermal Stability

Maintains over 60% of its activity for 2 hours at 70°C.

The microbial Swissaustral laccase shows higher activity at temperatures above 40°C when compared to fungal laccase from competitors.

Main Properties

Origin Thermophilic microorganism
Production Source Escherichia coli
Molecular Weight ~59 kDa
Activity ≥ 300,000 U/mg of protein
Unit Definition One unit is defined as a change in absorbance of 0.001 measured at 530 nm per minute, at 70°C and pH 6.0 using syringaldazine as a substrate.
Temperature Range 30–100°C (optimal: 80°C)
pH Range 5.5–7.5 (optimal: 6.0)
Other Components 0.05 M Tris Buffer pH 8 and 0.85 M NaCl
Storage Conditions At 20°C, it maintains the reported activity (≥ 300,000 U/mg of protein) at least for 2 years.

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